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University
of Minnesota
Mouse Microarray Group |
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| 6-04-03 BMAP Files | |
| Array Picture |
A
jpeg image of the last chip printed. Probed with a Genisphere 3DNA feature
detection probe, and scanned on the Scanarray 5000.
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| Spot Layout |
A
comma-delimited spreadsheet showing the visual layout of spots on the
microarray. Download and open using the (File...Open...) command
within MS Excel.
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| Full Sample Info |
A
tab-delimited spreadsheet containing more extensive information on the
genes spotted in the array. Download and open using the File...Open...)
command within MS Excel.
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| QuantArray Import |
A
plain-text file used to tell the QuantArray software where samples are
located in the array. Specify this file as the "Gene Database"
in QuantArray. Genes are identified by their 96-well plate location in
the BMAP set. (5c10 indicates plate 5, well c10)
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A
file in Axon's .gal format that tells GenePix software where samples are
located in the array, and how to set up the quantification grids.
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Spotting
Details
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Slide
orientation description:
The letter"C" is etched on the bottom right corner of the spotted slide and two parallel lines are etched on the back of the slide to mark the boundary of the spotted DNA. Your analysis protocol should be set up with 48 sub-arrays (4 columns by 12 rows) with 4.5mm between each array. Each array is a 22x22 grid with 0.2mm between spots and spot size around 170 microns. Printed at approximately 72 deg F and 47% humidity. |
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