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University
of Minnesota
Mouse Microarray Group |
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| 6-19-01BMAP Files | |
| Array Picture |
A
jpeg image of the last chip printed. Probed with a Genisphere 3DNA feature
detection probe, and scanned on the Scanarray 5000.
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| Spot Layout |
A
comma-delimited spreadsheet showing the visual layout of spots on the
microarray. Download and open using the (File...Open...) command
within MS Excel.
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| Full Sample Info |
A
tab-delimited spreadsheet containing more extensive information on the
genes spotted in the array. Download and open using the File...Open...)
command within MS Excel.
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| QuantArray Import |
A
plain-text file used to tell the QuantArray software where samples are
located in the array. Specify this file as the "Gene Database"
in QuantArray. Genes are identified by their 96-well plate location in
the BMAP set. (5c10 indicates plate 5, well c10)
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A
file in Axon's .gal format that tells GenePix software where samples are
located in the array, and how to set up the quantification grids.
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Spotting
Details
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Slide Orientation Description: The letter "C" is etched in the bottom right corner of the spotted side of the slide, and two parallel lines are etched on the back of the slide to mark the boudary of the spotted DNA. |
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Your analysis protocol should be set up with 48 sub-arrays of 16 columns by 16 rows with 0.28 mm spacing between spots. Contains entire BMAP library (that has been successfully amplified by PCR) and a variety of control genes, printed on the array once each. Printed at approximate temp of 76 deg. Fand approximately 46% relative humidity. |
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