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Veterinary Care/Special Services |
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Copyright 2005, University of Minnesota Board of Regents. The University of Minnesota is an equal opportunity educator and employer. |
Testing Cell Lines for Rodent VirusesA variety of infectious agents in rodents can affect the animals’ health and alter research results. Thus they are excluded from specific pathogen free (SPF) rodent colonies. Some of these agents, such as Mycoplasma species and various viruses can be cell culture or tumor cell contaminants. Therefore it is essential that any biologic products that are to be injected into SPF mice or rats are free of infectious agents. Unless cell lines are purchased from a commercial source that guarantees its produces are free of contaminants, and they are handled an aseptic manner, away from conventionally housed rodents that may carry the agents, contamination is a potential problem.Cell lines are tested for contamination using either the Mouse Antibody Production (MAP) test or PCR based tests. PCR testing is the preferred method used by RAR. To obtain testing contact RAR at 624-9100:
MAP testing is available through Charles River Laboratories for a cost of $425 per cell line.
The information contained in this site is intended as a reference for University of Minnesota investigators, and animal husbandry and veterinary staff. Drug information and dosages are derived from a variety of sources and do not necessarily guarantee safety or efficacy. Information obtained through this site should not be relied upon as professional veterinary advice. Any medications administered or procedures performed on animals should only be performed by or under order of a qualified, licensed veterinarian. |