| Pathogen
screening
Biosafety
Solid tumors
Hybridomas
Copyright 2000, University of Minnesota Board of Regents.
The University of Minnesota is an equal opportunity educator
and employer. |
 Guidelines
for Maintenance of Tumor Cell Lines and Hybridomas for Ascites Production
in Rodents
The implantation of tumors into
animals is a procedure that has the potential for causing pain and distress.
These guidelines are established by the University of Minnesota Institutional
Animal Care and Use Committee to address these concerns.
1. Cell lines should be tested
for the presence of murine viruses prior to introduction into the animal
colony.
2. Human cell lines should
either be certified free of human pathogens, or all work should be performed
according to Biosafety
Level 2 precautions. Workers should be offered the hepatitis
B vaccine through Boynton Health
Service.
3. Solid
Tumors
-
Mice inoculated with solid tumors
should be observed at least three times weekly to assess their physical
condition.
-
Animals with tumors that have
ulcerated or that interfere with the animals normal activity, or animals
that become emaciated or debilitated will require euthanasia.
4. Intraperitoneal implantation
of Hybridomas
There are numerous in
vitroalternatives to the in vivo
mouse ascites induction method of producing monoclonal antibodies.
These should be investigated prior to making a decision to use the in
vivo method. Check here for information.
Priming
-
0.5 ml is the preferred maximum
volume of pristane, complete Freund's adjuvant, or incomplete Freund's
adjuvant for priming. Up to 0.75 ml may be used in large mice.
Monitoring
for Distress
-
Animals should be assessed at
least once daily for clinical condition and food and water intake
-
If the animal is in distress,
it should be euthanized (refer to Euthanasia Criteria below)
Harvesting
Ascites Fluid
-
The ascites fluid can be harvested
with a 20 gauge or smaller needle. New personnel should be trained using
anesthetized mice.
-
The animal should be monitored
later on the same day it has been tapped
Euthanasia
Criteria- An animal must be euthanized if it meets any of the following
criteria:
-
It is moribund
-
It is lethargic, anorexic, dehydrated,
or shows other evidence of significant illness or distress
-
The skin of its abdomen is gray/green
-
It is in respiratory distress
Top of
page
The information contained in this site is intended as
a reference for University of Minnesota investigators, and animal husbandry
and veterinary staff. Drug information and dosages are derived from a variety
of sources and do not necessarily guarantee safety or efficacy. Information
obtained through this site should not be relied upon as professional veterinary
advice. Any medications administered or procedures performed on animals
should only be performed by or under order of a qualified, licensed veterinarian. |
